If you are new to the use of sterile filter tips, you may be wondering what makes them so important. Here are a few tips to help you decide which tip to buy and when. They can save you time, money, and health concerns. Choosing the right filter tip for your application is important, but remember that the correct fitting tip doesn't have to compromise your lab work. How to sterilize pipette tips?
When choosing a filter tip, make sure you choose one that is certified as sterile. If the label says pre-sterile, that means the product was already sterile when it left the manufacturer. If it's not, you might be using a contaminated tip or end up with a contaminated sample. In both cases, you may want to purchase a new tip. The quality of the sterile filter tip you purchase is important because the quality can affect the safety of your laboratory.
If you're looking for a sterile filter tip, Oxford Lab Products makes a number of high-quality options. Its patented low-retention polymer prevents aerosolized particles from entering the tip cone. It's compatible with many pipettes and comes in racks of 96 tips. Each rack contains 96 tips, so you'll never run out! The patented system is designed to provide maximum safety for your lab and reduce risk of cross-contamination.
Maintaining sterility is critical for many clinical and academic research applications. Microbial contamination can produce erroneous results, costly delays, and laborious troubleshooting. Whether you use sterile pipette tips or other instruments, it's vital to follow proper sterile practices to avoid contaminated consumables. The use of sterile pipette tips will help you perform your experiments in the safest and most reproducible manner possible.
A few key benefits of filter pipette tips include their high permeability, which is an important consideration when using air displacement pipettors. They are ideal for DNA amplification, forensics, tissue culture, and protocols involving radioactive solutions. They are also non-self-sealing, meaning you won't lose samples if you accidentally over pipet a sample. A variety of micro-pipette tips are designed to fit different pipettor types, and can reduce cross-contamination.
In addition to their sterile benefits, these tips also have many practical applications. They are commonly used in laboratories for loading agarose gels, isolating DNA, and many other non-sensitive applications. They are inexpensive and can be purchased in bulk or pre-racked, and have convenient reload options. Non-sterile tips are generally non-refillable, but can be sterilized with an autoclave.
Choosing the Right Pipette Tip
Using the wrong tip for a pipette can ruin the precision of your experiments. Not only can it cause contamination, but it can also lead to repeated stress injury and waste of reagents and samples. To help avoid these problems, here are some tips for choosing the right tip for your pipette. You can use any of the following types of tips:
High-quality filtered tips are made from high-grade polypropylene, which is free from chemicals and additives. They are also equipped with a non-self-sealing PE filter, which makes it easier to recover samples without risking contamination. High-quality filtered tips come in different volume sizes and are certified RNase and DNase-free. In addition, they are GLP compliant.
Non-filter/non-barrier pipette tips are useful for many applications, including those without a high degree of sensitivity. They're often used in laboratories to load agarose gels or isolate DNA. These tips are less expensive and come in bulk, pre-racked or convenient reloads. They're also not sterile, so you'll need to sterilize the storage boxes with an autoclave.
The type of polypropylene used to produce the tips is another factor to consider. Quality tips are made from virgin polypropylene and are free of plastic and metal additives. It's also important to check the shape of the tips to make sure that they're free of metal particles, as these can affect the precision and accuracy of your experiments. Finally, ensure that your tips are easy to fit and don't make your lab work more difficult than it needs to be.
Filter pipette tips have a filter at the proximal end of the tip to protect against aerosols, aspirates and damaging liquids. As a result, they're generally cheaper than barrier tips. Usually, barrier tips are only available in expensive versions. The filters slow down the flow of liquid into the pipette barrel, extending the life of the pipette.
Sterile pipette tips also eliminate the risk of contamination from aerosols. Pipettes can introduce a variety of contaminants into liquid samples, and contaminants can cling to them. In some cases, these contaminants affect enzyme activity or longevity, or may even interfere with chromatographic or spectrophotometric analysis. For these reasons, it's important to use certified tips whenever possible. You'll be glad you did.
When choosing a tip, you must consider the volume and dispense channel. A standard tip with a small orifice can tear cellular samples. A wide bore tip will prevent the risk of this by reducing flow resistance. Moreover, a wide-bore pipette tip will prevent the risk of damage to cellular samples. And as always, it's crucial to use sterile pipette tips for your experiments.